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Early Lenses
Alhazen
Title page of the Latin translation of Alhazen's Book of Optics (1021).
While van Leeuwenhoek was more of an observer than a theoretician, he owed two things to an Arabic-speaking Muslim, who lived in Egypt, Alhazen (965-1039). (His name is spelled various ways and his origin is either Arab or Persian.)
Alhazen used a method of obtaining new knowledge that we now see was scientifically sound. He was probably the first to use it so rigorously, and six hundred years later, as refined by Francis Bacon, van Leeuwenhoek was using it, too.
Like van Leeuwenhoek, Alhazen worked with lenses and he applied mathematics to understand them. Alhazen contributed to what we now see as a wide variety of scientific specialties, especially optics. Based on his experiments and his confidence in the conclusions he drew from them, Alhazen contended that light was reflected into the eye from the things observed. While this new idea was not as threatening as Galileo's five hundred years later, it was equally revolutionary by reversing a thousand years of wisdom from the ancients.
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Roger Bacon
Roger Bacon (c. 1214–1294), the English Franciscan friar, like Alhazen before him and van Leeuwenhoek after him, placed primacy in empirical reasoning from accurate observation. He was also fascinated with how lenses could both distort reality and make its observation more accurate, for example, a device with which "a child might appear a giant, and a man a mountain".
Bacon described the properties of magnifying glasses and in this illustration, the effect of a glass of water acting as a lens. The same phenomenon that was referred to by Robert Hooke in the 1600's and Bernard Cohen in the 1900's to explain some of van Leeuwenhoek's greatest magnifications. He was seeing things that it should not be possible to see with the best of his surviving lenses. Did he have better lenses? Or did he amplify their power by viewing through a thin glass tube acting as a lens, as Hooke suggests? Or did he focus on the wall of an air bubble on which his little animalcule happened to be living?
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Grinding
Spring-pole
lathe
Van Leeuwenhoek mentions his lathe in only one letter, October 9, 1676, the letter with the claims about "so many animalcules" that the Royal Society went to such lengths to validate (see Science page):
Note, that my Study stands toward the North-east, and is partitioned off from my antechamber with pine-wood, very close joined, having no other opening than a slit an inch and a half broad and 8 inches long, through which the wooden spring of my lathe passes.
This "wooden spring" is the springy pole in the drawings, suspended from the ceiling. The design had not changed for hundreds of years. In van Leeuwenhoek's house, it seems from this letter that the pole was attached in the antechamber and passed through a vertical slit in the wall into his study, where he did his grinding, if not his observing. The antechamber may well have been part of his shop.
From end of the pole, like a fishing pole, hung a line wrapped around the lathe's axle. Van Leeuwenhoek would push it down with his foot and then let up. The pole would spring back, and he would press down with his foot again, creating a back and forth motion for his grinding of lenses or turning of screws.
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Glass
to grind
A lens can be only as good as the glass it came from, so van Leeuwenhoek probably used Venetian or Murano glass made in the Netherlands with techniques imported from Italy. Van Leeuwenhoek would have used a low-power magnifying glass to inspect the shards for defects.
Warm balsam glues were common at the time, and still work.
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Grinding
the glass
Although a mold can be pressed onto a turning piece of glass, it is more likely that van Leeuwenhoek used a foot treadle to turn the mold and then pressed the glass into it.
The photo shows a vertical lathe but van Leeuwenhoek probably used a horizontal lathe.
To make his convex lenses, he would need a hard mold or bit in the reverse, that is, concave, shape. The concave mold could have been iron, brass, copper, bone or pewter or even a hardwood like beech, all of which were commonly used at the time. He would coat it with successively finer abrasives as he ground it smaller and then smoother.
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Flat
or round
Even if he blew or melted glass to make the lens blanks, van Leeuwenhoek could have then used the lathe either to flatten or smooth the surface or to increase the curvature.
There is no reason to assume that van Leeuwenhoek always made every lens with the greatest magnification and highest resolution. These lenses were so small that their focal length is measured in tenths of millimeters. For many of his observations, a weaker lens would provide the level of detail that he needed without having to move the specimen out of the focal length.
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Blowing
glass blower
Luyken glass blower
Until van Leeuwenhoek's time, the artisans of Venice made the best glass and the rulers of Venice went to great lengths to keep the techniques and ingredients secret. However, in 1612, Antonio Neri wrote and published L'arte vetraria, The art of glass, in which he spilled all the secrets.
A priest, Neri was born (1576) and died (1614) in Florence but spent most of his short adult life in Antwerp. His book revealed recipes and techniques for materials, furnaces, temperatures and melting times. One of his own discoveries was ruby gold or cranberry glass, made from adding a bit of gold to the molten liquid.
Neri's publication in Italian, its translation into Latin, and by 1662, Christopher Merrett's translation into English with the subtitle, "Wherein are shown the wayes to make and colour Glass, Pastes, Enamels, Lakes, and other Curiosities", made high-quality glass readily available throughout Europe. Merrett's notes doubled the length and updated the content enough that the book became the bible of glassmaking until the 19th century.
While we have no direct evidence that van Leeuwenhoek knew of Neri's work, its fame and van Leeuwenhoek's own lens making, which improved over time, indicate that he profited from the spread of this knowledge.
Merrett was a founding Fellow of the Royal Society.
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In several letters, van Leeuwenhoek speaks of "blowing glass," and he apparently used that technique for many years in the middle of his career. He would have melted one end of a glass tube and blown into the other to make a bulb at the far end. Or he would have closed off a glass tube at both ends. The expanding air inside will form enough of a bulb at one end.
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To create sufficient heat, van Leeuwenhoek would have burned a vegetable oil and have used a foot-bellows. He would have melted the tip down to a bead that he would chip off.
Van Leeuwenhoek seemed to put more effort into making his lenses than into the metal parts, more emphasis on the optics than the mechanics, which were just good enough to keep the lens and specimen very close together.
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After the
tip was chipped off, the flat side was ground and polished smooth to
make a plano-convex lens or a convex-convex lens.
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Drawing
Heat a
glass rod in the center (top). Slowly pull it apart (center) until it
gets very thin, less than 1 mm. Then break it. Slowly feed one filament
back into the flame (bottom), continually twirling it until a small
spherical bead forms on the end.
On a drop of molten glass that small, the surface tension tends to be
uniform and sufficiently larger than the mass of the glass. Thus, it
tends to form a smooth sphere that will need minimal, if any, grinding
and polishing.
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In
Micrographia, published in 1665,
ten years before Leeuwenhoek started
publishing his research, Robert Hooke described this technique:
If you take a very clear piece of a broken Venice Glass, and in a Lamp
draw it out into very small hairs or threads, then holding the ends of
these threads in the flame, till they melt and run into a small round
Globul, or drop, which will hang at the end of the thread.
Hanging it off the end as in the illustration will let gravity make a
slightly aspherical shape. Twirling the filament, what Hooke calls
"hairs or threads", will help keep the shape more spherical.
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Hooke
continues:
And if further you stick several of these upon the end of a stick with
a little sealing Wax, so as that the threads stand upwards, and then on
a Whetstone first grind off a good part of them, and afterward on a
smooth Metal plate, with a little Tripoly, rub them till they come to
be very smooth.
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Hooke
concludes his section on this type of microscope:
If one of these be fixt with a little soft Wax against a small needle
hole, prick'd through a thin Plate of Brass, Lead, Pewter, or any other
Metal, and an Object, plac'd very near, be look'd at through it, it
will both magnifie and make some Objects more distinct then any of the
great Microscopes.
But because these, though exceeding easily made, are yet very
troublesome to be us'd, because of their smallness, and the nearness of
the Object; ....
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Spermatozoa
After
discovering what he called "seminal animalcules" in the late 1670's,
Van Leeuwenhoek spent the next forty years finding, describing,
comparing, and speculating about them in thirty kinds of animals:
7 mammals
2 birds
1 amphibian
7 fish
11 arthropods
2 mollusks
His letters of November 1677, and March 18, 1678,
included drawings of both human and dog sperm.
These photographs of human sperm are shown here at about the
magnification of van Leeuwenhoek's better lenses.
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Drawings
from van Leeuwenhoek's letter of March 18, 1678.
Figures 1 through 4 - rabbit sperm
Figures 5 through 8 - dog sperm
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Drawings
of rabbit sperm taken from van Leeuwenhoek's letters.
Recently a little book
is fallen into my hands, ... in which among
others is said: But the most strangest story is, that the scholar Mr.
Cornelis Bontekoe told us (on good authority) from the curious
Leeuwenhoek, that the human sperm is abundant of little infants and so
on in the nature of things.
Indeed, it is true that Mister Bontekoe has visited me several times;
However, I never have used such arguments to him, nor to anybody in the
world, namely that human sperm is abundant of little infants: but I did
say that there were plenty of living animals or worms in it, having
long tails, and it is just that construction I showed in the figure.
While he had no conception of DNA, van Leeuwenhoek knew that sperm did
not contain
pre-formed individuals.
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Drawing
of sperm from a ram.
Little animalcules that
moved forward with a snaillike motion of the
tail.
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Blood
This
image of an "unmounted and unstained blood smear" was taken by Brian
Ford looking through the authentic van Leeuwenhoek microscope at the
University of Utrecht. It magnifies about 255 times and has enough
resolution to clearly show the erythrocytes.
Note the irregularities of the hole van Leeuwenhoek punched in the
brass as well as the spherical aberration around the edges.
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An
enlargement of the best-resolved upper right of the Brian Ford blood
smear viewed through the authentic Utrecht microscope.
Note the polymorphonuclear
leukocyte's lobed nucleus in the upper right.
Van Leeuwenhoek did not note any leukocytes in his letters, but this
lobed nucleus shows the resolving power of his lenses.
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Tiny Lenses
The three methods van Leeuwenhoek used
to make his lenses:
grinding, blowing, and drawing.
First came eyeglasses. For centuries after Alhazen and Roger Bacon first described lenses, people ground crude glass lenses to aid sight. Magnification of two or three times was usually sufficient. In addition, the human eye doesn't need very much of the lens at any given time, so the lens does not need overall uniformity.
Then came telescopes. In the first half of the 1600's, Galileo and others used two lenses to develop powerful telescopes, convex objective (closer to the specimen) and concave ocular (closer to the eye). Other telescopes, following Kepler's design, had a convex objective and a convex ocular. The stars, planets, and moons were very far away, so the larger the lens, the better. Also, the farther apart the lenses, the less the chromatic aberration.
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These early astronomers soon realized that the telescope could be turned around, from the skies above us to the world around us, from the very far away to the very close.
First microscopes
The first microscopes, such as Galileo's in 1609, were just little telescopes with a concave lens on one end of a tube and a convex lens at the other end. After the discovery that two convex lenses would work better for microscopes, the best double-lens microscopes of the 1600's magnified twenty to thirty times to a resolution of eight microns.
However, they suffered from inherent aberrations, both chromatic and, more importantly, spherical.
Single lenses
What happens when you use only one lens?
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Specifically, take two plano-convex lenses -- flat on one side and curved out on the other. Put them back to back to create a transparent sphere. In that case, the greater the arc, that is, the greater the angle of the curve along the outside, the greater the magnification. In the illustration below right, the brown sphere, half the diameter of the pink sphere, clearly has a greater curvature. If you don't quite see that, take it to the extreme in the other direction: the earth is such a big sphere compared to you that its surface appears to stretch flat as far as you can see.
What Leeuwenhoek and many others discovered was that the smaller the lens, the greater the radius of curvature and the greater the magnification.
The microscope with a single convex-convex lens, that is, a sphere, had greater powers of magnification than the double-lens microscopes. The strongest of the nine surviving van Leeuwenhoek microscopes magnifies 266 times to a resolution of a little more than one micron. Most of the rest magnify more than a hundred times to a resolution of three or four microns.
However, the tiniest single lenses were very difficult to make and even more difficult to use.
Van Leeuwenhoek's lenses
The two most adept at making these tiny lenses were Robert Hooke in England and Antony van Leeuwenhoek in the Dutch Republic. How did they make and use these tiny lenses? What made them so far ahead of their time?
Van Leeuwenhoek made at least 500 microscopes, apparently one for each specimen that he wrote about, an average of almost one per month over the fifty years of his research. Depending on what he was observing, less powerful lenses were often more effective.
He wrote on June 9, 1699:
Concerning my Self, although they have been made by me for these Forty Years almost, on an extraordinary smallness, yet they have been but little used by me; for according to my judgment, they are not fit to make the first Discoveries, for these that are ground of a bigger Diameter, are more fit for that.
We still have a dozen of his hand-made spherical lenses, most of them smaller than 3 millimeters. The best, that is, the smallest, has a diameter of less than 1.5 millimeters, about 5 hundredths of an inch. They were made with the standard Venice glass of the time, full of imperfections that would distort his observations.
Grinding
Grinding is the traditional way to make a small lens. In van Leeuwenhoek's time, glass grinding techniques were based on the centuries-old technique of grinding lenses for eye-glasses (spectacles). Grind a piece of glass under a cap or mold coated with increasingly fine material. Then polish it. The tiny cap containing the material, the grinding sand or the polishing paste, fits over the glass.
Van Leeuwenhoek used a spring-pole lathe to make the screws for his microscopes as well as the lenses. Foot power with a spring pole gently turns the cap around the glass. Van Leeuwenhoek's pole was so big that he had to cut a hole in the wall. He wrote in 1676:
My Study stands toward the North-east, and is partitioned off from my antechamber with pine-wood, very close joined, having no other opening than a slit an inch and a half broad and 8 inches long, through which the wooden spring of my lathe passes.
For more, see the lathe pop-up under Grinding in the left-hand column.
All of the surviving dozen van Leeuwenhoek lenses are practically spherical. All but one of them were ground, but we do not know how representative they are of all the lenses he made.
Blowing
Blown lenses were also popular. A little blob of hot glass can be fashioned at the end of a blown piece of glass. These lenses will be plano-convex, flat on one side and curved out on the other. The flat side, knocked off the larger piece of glass, could be ground flat using the grinding lathe technique above.
None of van Leeuwenhoek's surviving lenses is plano-convex. However, in letters, he mentions blowing his lenses.
[ AvL quote needed ]
Drawing
In Micrographia, in 1665, Hooke described a third method, in addition to grinding and blowing: how to pull or draw a glass rod to make a lens.
Take a very clear piece of a broken Venice Glass, and in a Lamp draw it out into very small hairs or threads, then hold the ends of these threads in the flame, till they melt and run into a small round Globul, or drop, which will hang at the end of the thread.
Hanging it -- the drop, globule, or bead -- off the end will let gravity make a slightly aspherical shape. Twirling the filament, what Hooke calls "hairs or threads", will help keep the shape more spherical.
Of the surviving van Leeuwenhoek lenses in microscopes, most are ground and one, the best, was made from a melted drop.
Birch's History records that on March 14, 1678, Hooke showed the members of the Royal Society how he made the lens that he used to finally replicate van Leeuwenhoek's results.
After which he [Hooke] shewed the method, by which he made two sorts of microscopes, and the conveniences and inconveniences of both these.
The first was a single microscope made by a small globule of glass, by means of which, with very little or no difficulty, any object might be prodigiously magnified. He also explained how the globule was made out of a thread of glass, and how that glass thread and small glass-canes were made.
The drop could stay on a bit of its thread and the whole thing mounted between the metal plates to make a microscope. Or the thread could be ground off and the drop smoothed out using the lathe grinding techniques above. In that case, the drop could be said to be ground even though its refracting surfaces were not.
Hooke would expand on these ideas in his response to van Leeuwenhoek's letters on October 5, 1677, and January 14, 1678, all of which he published in his book Microscopium.
In the following year, Mr. Butterfield had a letter published in Philosophical Transactions, volume 12 (.pdf) , "about the making of Microscopes with very small and single Glasses".
I Doubt not but you may be as busie at London as we are here in making of Microscopes of the manner lately brought out of Holland by Mr. Huygens, where I have of several fashions ready made. -- using Spirit of wine instead of tallow candle or wax.
The Mr. Huygens mentioned here is Christiaan Huygens, a prominent member of the French Royal Academy and the person responsible for excerpts of van Leeuwenhoek's letters being translated from the English in Philosophical Transactions to French for Journal des Scavans. Without mentioning van Leeuwenhoek, Butterfield continues (emphasis added; more on As Science Began page):
Then take your beaten Glass, being first washed very clean, upon the point of a Silver needle filed very small, and wet with spittle. Hold it thus in the flame till it be quite round, and no longer for fear of burning it, and if the side of the Glass next to the needle be not melted, you may put it off an take it up with the needle on the round side, presenting the rough side to the flame till it be every where very round and smooth, then wipe and rub one or several of them together with soft leather, which makes them much the better.
Then put them between two pieces of thin brass, the Apertures very round and without bur, and that towards the eye so big almost as the diameter of the Glass: and so placed in a Frame with the object conveniently for observation.
Compared to the plano-convex lens from blowing, this method produces spherical lenses. Compared to grinding, this method produces smoother, less scratched lenses, "every where very round and smooth", according to Butterfield. Plus, you can make dozens of these tiny lenses in an evening and then use a larger low-powered lens to see which of the tiny lenses are the most spherical and free of imperfections.
Then van Leeuwenhoek had a new problem. For a lens so small, the specimen must be so close as to almost touch the lens, as must the observer's eye. How can the lens best be positioned between the specimen and the eye? How does the tiny lens become a useful magnifying glass?
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Magnification
lens types
Lens have two sides and three shapes, convex, concave, and plane (straight). Putting two plano-concave lenses back-to-back produces the diverging bi-concave lens on the right. Putting two plano-convex lenses back-to-back produces the converging bi-convex lens on the left, which is what van Leeuwenhoek used. He increased the angle of curvature until it became a sphere.
If the glass is straight and smooth, if it has no curvature, then the light passes straight through without distortion or magnification.
The simplest magnifying lens is the bi-convex convergent lens (sometimes called double-convex) that condenses light rays into a focal point. The focal length of a bi-convex lens depends on the curvature angle of its faces. Higher angles of curvature result in shorter focal lengths because the glass refracts (bends) the light waves at a greater angle from the centerline of the lens. The symmetric nature of bi-convex lenses minimizes spherical when the image and object are symmetrical distances apart. These lenses are typically used for focusing and image magnification.
Given the constraints of glass, grinding techniques, and the size of the human eye, van Leeuwenhoek's smallest lenses, a millimeter or two in diameter, were as small as pratical and possible.
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spherical lens
As you can see on this diagram, when the glass is curved, the light angles up. So when you're in the blue part looking through, the object appears larger than if the ray passed straight through. Clearly then, the more you increase the curvature, the more you increase that angle, and the greater the magnification.
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Lens Aberrations
Spherical
aberration
Blurry
images were one of the chronic problems faced by early users of
telescopes and microscopes.
The technical term is spherical aberration. Flat glass has no such
problem, but for a lens with a spherical surface, light rays enter
parallel to the optical axis. But those rays which are farther from the
center fail to converge to the same point.
Van Leeuwenhoek's microscope design of mounting the lens in an
aperture slightly smaller than the lens not only held the lens. It
reduced the spherical aberration
by eliminating the light rays coming
from the edges.
The better solution, non-spherical lenses, was not possible until the
1800's.
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Chromatic
aberration
The other chronic problem facing early users of telescopes and microscopes was chromatic aberration, a fringe or halo of false color. It occurs because short wavelengths bend more than long wavelengths, and thus different colors focus at different distances from a lens. Chromatic aberration remained a serious problem into the 1800's.
Chromatic aberration is also compounded by multiple lenses. Kepler re-designed his equi-convex lens to make one surface shallower, even planar, to slightly reduce the chromatic aberration. Another solution was to increase the focal length, leading to absurdly unwieldy contraptions. A telescope will scale only so far.
Another improvement was made by Christiaan Huygens, who added a field lens near the eyepiece lens. They were both plano-convex lenses with the plane sides towards the eye. In addition to reducing chromatic aberration, this double eyepiece provided a wider field of view by re-imaging the objective. The microscopes that Hooke designed used Huygen's double eyepiece.
Leeuwenhoek's spherical lens is an extreme form of Kepler's equi-convex lens.
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Bacteria
Van
Leeuwenhoek's letter of February 9, 1702, describes cyanobacteria (aka
blue-green algae).
On the 25th of August, I saw that in a leaden gutter, on the front of
my house, for a length of about five feet and a breadth of seven
inches, some rain-water had remained standing, which had a red color.
... I took a drop or so of this water and looked at it through the
microscope; and I discovered a great many animalcules that were red,
and others that were green. ...
These animalcules were for the most part round, and the green ones
were somewhat yellowish in the middle of their bodies.
Their bodies seemed to be composed of particles that presented an oval
figure; and therewithal they had short thin instruments which stuck out
a little way from the round contour, and wherewith they performed the
motions of rolling around and going forward; and when they took a rest,
and fixed themselves to the glass, they looked like a pear with a short
stalk; but this stalk, on curious examination, was split at the end, or
divided into two, and twas with these two parts that the animalcules
fixed 'emselves flat to the glass.
Clifford Dobell, microbiologist and van Leeuwenhoek biographer,
states that this passage refers to Hematococcus pluvialis.
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In his
letter of January 14, 1678, van Leeuwenhoek wrote:
I can't help but mentioning that I can now make out, very plain and
clear, the shape of those little animals of the smallest sort, whereof
I said before that I could ascribe no figure to them; and this because
of the pleasure that I do take in their manifold delightful structures,
and the motion that they make from time to time in the water. ... These
would oft-tmes shoot so swiftly forward with the hindmost part of their
body, that you might think you saw a pike darting through the water.
Clifford Dobell, microbiologist and van Leeuwenhoek biographer,
suggests that this passage refers to Spirillum genus of bacteria.
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On
September 17, 1683, Leeuwenhoek wrote:
Furthermore, the most part of this matter that I took from between my
front teeth consisted of a huge number of little streaks, some greatly
differing from others in their length, but of one and the same
thickness withal; one being bent crooked, another straight, like Fig.
F, and which lay disorderly ravelled together.
And because I had formerly seen, in water, live animalcules that had
the same figure, I did make every endeavour to see if there was any
life in them.
Clifford Dobell, microbiologist and van Leeuwenhoek biographer,
suggests that this passage refers to Leptothrix buccalis.
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On
September 4, 1674, Leeuwenhoek wrote:
About two hours
distant from this Town there lies an inland lake,
called the Berkelse Meer, whose bottom in many places is very marshy,
or boggy. Its water is in winter very clear, but at the beginning or in
the middle of the summer it becomes whitish, and there are then little
green clouds floating through it; which, according to the saying of the
country folk dwelling thereabout, is caused by the dew, which happens
to fall at that time, and which they call honey-dew. This water is
abounding in fish, which is very good and savoury. Passing just lately
over this lake, at a time when the wind blew pretty hard, and seeing
the water as above described, I took up a little of it in a glass phial;
and examining this water next day, I found floating there in diverse
earthy particles, and some green streaks, spirally wound serpent-wise,
and orderly arranged, after the manner of the copper or tin worms which
distillers use to cool their liquors as they distil over.
The whole circumference of each of these streaks was about the
thickness of a hair of one's head. Other particles had but the
beginning
of the foresaid streak; but all consisted of very small green globules
joined together: and there were very many small green globules as well.
Clifford Dobell, microbiologist and van
Leeuwenhoek biographer,
suggests that this passage refers to cyanobacteria from the Spirogyra genus.
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Protozoa
According
to Clifford Dobell, microbiologist and van Leeuwenhoek biographer, van Leeuwenhoek was the first person to see the
bdelloid rotifer Philodina roseola.
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According
to Clifford Dobell, microbiologist and van Leeuwenhoek biographer, van Leeuwenhoek was the first person to see the
bdelloid rotifer Philodina roseola.
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Van
Leeuwenhoek's letter of December 25, 1702 described a stalked inverted
bell-shaped ciliates of the genus Vorticella,
according to Clifford
Dobell, microbiologist and van Leeuwenhoek biographer.
In structure these little animals were fashioned like a bell, and at
the round opening they made such a stir, that the particles in the
water thereabout were set in motion thereby. ...
And though I must have
seen quite 20 of these little animals on their long tails alongside one
another very gently moving, with outstretched bodies and
straightened-out tails; yet in an instant, as it were, they pulled
their bodies and their tails together, and no sooner had they
contracted their bodies and tails, than they began to stick their tails
out again very leisurely, and stayed thus some time continuing their
gentle motion: which sight I found mightily diverting.
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Van
Leeuwenhoek's letter of October 6, 1676, reported that, earlier in the
year, he had seen the ciliate of the genus Colpidium,
according to
Clifford Dobell, microbiologist and van Leeuwenhoek biographer.
The 23rd of May, I
discovered, besides the foresaid animalcules,
living creatures that were perfectly oval, like plovers' eggs. I
fancied that the head was placed at the pointed end, which at times was
stuck out a bit more. Their body within was furnished with some 10, 12,
or 14 globules, which lay separated from one another.
When I put these animalcules on a dry place, they then changed their
body into a perfect round, and thereupon oft-times burst asunder; and
the globules, together with some watery humour, flowed out on all
sides, without my being able to discern any other remains.
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Van
Leeuwenhoek's letter of November 4, 1681, described the flagellated
protozoan parasite Giardia lamblia, according to
Clifford Dobell, microbiologist and van
Leeuwenhoek biographer.
... a clear transparent medium, wherein I have sometimes also seen
animalcules a-moving very prettily; some of 'em a bit bigger, others a
bit less, than a blood-globule, but all of one and the same make.
Their
bodies were somewhat longer than broad, and their belly, which was
flatlike, furnisht with sundry little paws, wherewith they made such a
stir in the clear medium and among the globules, that you might even
fancy you saw a pissabed running up against a wall; and albeit they made
a quick motion with their paws, yet for all that they made but slow
progress.
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From his
drawings and descriptions, we can see that van Leeuwenhoek
was the
first person to see what we now call single-celled eukaryotes
or
unicellular heterotrophic protists, aka protozoa.
While he had no names for them and the drawings accompanying his
descriptions are not always clear, we can see now that he discovered
dozens of protozoa. Pictured here (top to bottom) are the ciliates Oxytricha sp., Stylonychia mytilus,
and Euplotes, and Euglena. Van
Leeuwenhoek noted that when the water dries up, a Euglena forms a spore
and lies dormant until its environment improves.
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